Part:BBa_K5437022:Design

Controlled expression of MVA, MEP, GPP, L-borneol Biosynthesis Pathway Genes in pBAD

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2341
  Illegal NheI site found at 5734
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 9767
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1807
  Illegal NgoMIV site found at 2032
  Illegal AgeI site found at 3727
  Illegal AgeI site found at 8011
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 661
  

Design Notes

Three araBAD promoters placed with multiple RBS, one for each basic part.

Source

Composite of basic parts plus araBAD promoter from plasmid backbone pBAD.